Transferrin 抗体
Quick Overview for Transferrin 抗体 (ABIN7661012)
抗原
See all Transferrin (TF) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Thomsen-Friedenreich Antigen / CD176 (Pan Carcinoma Marker)(A78-G/A7)
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产品特性
- This antibody recognizes a disaccharide epitope, Gal 1-3GalNAc, of Thomsen-Friedenreich (TF) antigen. It is specific for both anomeric forms of the disaccharide (TF and TF , including related structures on the glycolipid) and shows no cross-reactivity with sialylated glycophorin. The Thomsen-Friedenreich antigen acts as an oncofetal antigen, with low expression in normal adult tissues but increasing to fetal levels of expression in hyperplasia or malignancy. It is considered as a pan-carcinoma marker. This MAb is capable to agglutinate desialylated red blood cells. During metastasis, the ability of malignant cells to form multicellular aggregates via homotypic or heterotypic aggregation and their adhesion to the endothelium are critical. The tumor-associated carbohydrate Thomsen-Friedenreich antigen (Gal-GalNAc) is involved in tumor cell adhesion and tissue invasion. It also causes an immune response, and overexpression of the antigen causes cancer cells to be more sensitive to natural killer cell lysis. The Thomsen-Friedenreich antigen is suppressed in normal healthy cells and represents one of the few chemically well-defined antigens associated with tumor malignancy. The presence of the Thomsen-Friedenreich antigen on the surface of cancer cells may result from a divergence from the normal pathway for O-linked glycosylation in these cells, most likely caused by inappropriate localization of the enzymes involved in synthesis of the disaccharide. This antibody is available purified, with BSA and azide (0.2 mg/mL) or purified, BSA-free and azide-free (1 mg/mL).
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免疫原
- Neuraminidase-treated human red blood cells
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亚型
- IgM, kappa
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anti-Transferrin (TF) (AA 361-683) antibody
TF 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Transferrin (TF) antibodyTF 适用: Cow WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Transferrin (TF) antibodyTF 适用: Pig WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Transferrin (TF) (AA 22-176) antibodyTF 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Transferrin (TF) (AA 551-650) antibodyTF 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1C2 unconjugated
anti-Transferrin (TF) antibodyTF 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Transferrin (TF) antibodyTF 适用: 人 WB, IHC (p), IF (p) 宿主: 小鼠 Monoclonal 1F10 unconjugated
anti-Transferrin (TF) (AA 284-575) antibodyTF 适用: Cow WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Transferrin (TF) antibodyTF 适用: 人 WB, IHC (p), IF 宿主: 小鼠 Monoclonal 9B3 unconjugated
anti-Transferrin (TF) (AA 432-466) antibodyTF 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB55980 unconjugated
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应用备注
- Immunohistochemistry (formalin-fixed): 0.5-1.0 μg/mL for 30 minutes at RT. Immunofluorescence: 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: KG1 cells. Human colorectal carcinoma tissues.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS, 0.05 % BSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- Transferrin (TF)
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别名
- CD176
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背景
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Synonyms: T-F Antigen, TF Antigen, Asialoglycophorin, pan-carcinoma marker, CD176
Gene Symbol: Not Applicable
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分子量
- Multiple
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途径
- Transition Metal Ion Homeostasis
抗原
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